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KMID : 0382419990250010006
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Korean Journal of Environmental Health Society
1999 Volume.25 No. 1 p.6 ~ p.9
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Comparison of Sensitivity of Detection for Enterotoxigenic Escherichia coli Enterotoxin by use of the Reversed Passive Latex Agglutination and the Polymeras Chain Reaction
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Jung Hee-Kon
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Abstract
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Detection for heat-labile enterotoxin(LT) of enterotoxigenic Escherichia coli(ETEC) by use of the reversed passive latex agglutination(RPLA) was positive reaction from 2-fold(50 ng/ml) to 64-fold dilutions in EC81 and EC83. The polymerase chain reaction(PCR) which was using LT gene-specific primers of ETEC with a detection limit equivalent from 1 ng/㎕ to 1 pg of a DNA fragment of 417-bp in EC81 and EC83. The PCR provided a rapid and more sensitivity tool for the detecting LT of ETEC compare to the RPLA.
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KEYWORD
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